后期酰基转移酶编码基因lpxL对禽致病性大肠杆菌菌株E058致病性的影响

doi:10.11843/j.issn.0366-6964.2016.09.018

摘要/Abstract

摘要：

本研究旨在探讨禽致病性大肠杆菌后期酰基转移酶编码基因lpxL对其细胞膜结构、生物学特性的影响，揭示大肠杆菌的致病机制。通过构建大肠杆菌lpxL基因突变株，比较其与野生株的结构、生物学特性及致病性的差异。结果显示lpxL基因突变株的细胞表面结构较平滑，生长速度、内毒素含量与野生株相比明显下降；相应的LPS刺激HD11细胞后，细胞产生的NO及细胞因子表达量也较野生株LPS处理组有不同程度的下降；感染试验显示突变株对SPF鸡的脏器损伤明显减弱。综上所述，后期酰基转移酶编码基因lpxL对禽大肠杆菌的生物学特性及致病性十分重要。

Abstract:

The contribution of the gene lpxL to cell membrane structures and bionomics of avian pathogenic Escherichia coli (APEC) was studied to reveal the pathogenic mechanism of APEC to avian.An isogenic lpxL mutant was constructed from APEC O2 strain E058,and then compared its structure，biological characteristics，and pathogenicity with E058.The results showed that compared with E058，the lpxL mutant had a smoother surface，slower growth rate and reduction of endotoxin.Nitric oxide production reduction and cytokine gene transcription downregulation also were observed in HD11 treated with mutant-derived LPS relative to that in cells treated with E058-derived LPS at different times.The histopathological lesions in visceral organs of birds challenged with the wild-type strain were more severe than in birds infected with the mutant.These results indicated that the lpxL gene is important for the pathogenicity and biological activity of APEC strain E058.

中图分类号:

S852.612

许慧卿，高璐，穆晓惠，高清清，凌洁露，高崧. 后期酰基转移酶编码基因lpxL对禽致病性大肠杆菌菌株E058致病性的影响[J]. 畜牧兽医学报, 2016, 47(9): 1888-1896.

XU Hui-qing，GAO Lu，MU Xiao-hui，GAO Qing-qing，LING Jie-lu，GAO Song. Effect of the lpxL Lipid A Biosynthesis Pathway Gene on Pathogenicity of Avian Pathogenic Escherichia coli Strain E058 to Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1888-1896.

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